fraction method meaning in Chinese
分数法
Examples
- Profilometry based on the improved nonlinear excess fraction method
基于改进的非线性小数重合法的轮廓测量 - A surface antigen hemagglutinin - neuraminidase ( hn ) gene of the ndv b95 strain , was selected to study as target gene . referred to the reported sequence , rwo primers were designed and synthesized . hn gene of ndv b95 strain was amplified by rt - pcr , the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fraction method
为此，从澳大利亚某大学兽医病理系引进一株ndv热稳定性天然弱毒b95株，并选取其表面抗原血凝素?神经氨酸酶的编码基因为目的基因，根据国外已发表的序列，设计一对引物，利用rt - pcr技术扩增出了ndvb95株hn基因。 - In recent years , nonlinear methods have attracted more and more attention and there have been some successful cases , such as median filter , mathematical morphology , etc . as a preferred way to inverstigate nonlinear numerical problems , the continued fractions method can effectively express the gradually changing data or abrupt data , so it is meaningful to study image processing by means of the continued fractions theory and algorithms
近年来在图像处理领域，利用非线性方法进行图像处理取得较好效果的有中值滤波、数学形态学等，非线性方法已引起越来越多研究者的重视。作为研究非线性数值问题的首选方法?连分式方法，不仅能反映数据的渐变性，也能反映数据的突变性。鉴于这些原因，本文将连分式插值和逼近引入到数字图像处理领域，开展了图像插值、图像重建等方面的研究。 - Major difference of hn proteins lies in no . 18 - no . 75 amino acid residues on n - terminal which includes three active sites of hn protein . the influence on biological action , caused by the difference of hn protein , is needed to study further . hn gene has only four glycosylation sites , which is 1 or 2 less on amount than that of other strains . so , this difference can be take on as a natural mark of ndv b95 strain furthermore , the fragment encoding hn gene was excised from the positive clone pgem - hn with sail and saci enzyme , and purified by agrose gel fraction method . then the fragment was subcloned into the pet - 28c expressing vector digested by sail and saci restriction enzyme
作者将正确的重组克隆质粒pgem - hn用saii 、 saci双酶切，电泳回收目的片段，将其亚克隆到经saii 、 saci双酶切处理的pet - 28c中，转化大肠杆菌tgi感受态细胞，得到的转化子经pcr鉴定和酶切分析，筛选出符合阅读框的重组子，构建成重组表达质粒pet - hn ，并在大肠杆菌bl _ ( 21 ) ( de _ 3 )宿主菌中成功地表达了含目的蛋白的融合蛋白，融合蛋白的分子量约66kd ，加入iptg诱导8h后，蛋白表达几乎达到最高水平。